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As a biological macromolecule, mRNA can only be synthesized on a large scale by in vitro transcription (IVT). T7 promoter is currently one of the most efficient transcriptional promoters, so T7 RNA Polymerase (T7 RNA
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Image Search Results
Journal: PLOS Pathogens
Article Title: A novel ilarvirus protein CP-RT is expressed via stop codon readthrough and suppresses RDR6-dependent RNA silencing
doi: 10.1371/journal.ppat.1012034
Figure Lengend Snippet: (A) gfp-c3 was introduced by agroinfiltration into N . benthamiana leaves along with a plasmid encoding a candidate VSR (right half of leaf) or the empty plasmid (EP) (left half of leaf). (B) Agroinfiltrated N . benthamiana leaves were imaged under UV light at 4 dpi. The agroinfiltrated constructs are indicated above the images. (C) RT-qPCR analysis of gfp mRNA accumulation. Nb ACT-b (GI:380505031) was used as a reference gene. Values represent the mean +/− SD ( n = 4). (D) Northern blot analyses depicting the accumulation of gfp -specific siRNAs. A hydrolysed Cy5 labelled in vitro transcribed RNA fragment complementary to gfp was used as a probe. 5S ribosomal RNA was used as a loading control. (E–G) As for panels A–C except mgfp5 and dsGF were used instead of gfp-c3 .
Article Snippet: The Cy5 probe was generated using the
Techniques: Plasmid Preparation, Construct, Quantitative RT-PCR, Analysis, Northern Blot, In Vitro, Control, Fluorescence Microscopy